SFR Sensor Flask Reader
The SFR - Shake Flask Reader monitors oxygen and pH in up to 9 shake flasks simultaneously. It fits in nearly all standard shakers. The corresponding ready-to-use flasks contain pre-calibrated sensor spots. The system monitors non-invasively through the transparent bottom of the flask. Different types and sizes of flasks are available. Plastic flasks contain pre-calibrated oxygen and pH sensors. They are ready-to-use for one cultivation. Glass flasks are equipped with autoclavable oxygen sensors.
The SFR Shake Flask Reader monitors oxygen and pH in up to 9 Erlenmeyer flasks, cultivation tubes, or T-flasks simultaneously. It fits in nearly all standard shakers. Measurement data is transferred wirelessly via Bluetooth to your PC / notebook. The corresponding ready-to-use vessels contain pre-calibrated sensor spots. The system monitors non-invasively through the transparent bottom of the vessels. Different types and sizes of flasks and tubes are available. Plastic, disposable flasks contain pre-calibrated oxygen and pH sensors. Glass flasks are equipped with autoclavable oxygen sensors only, and can be re-used.
Features
- Rapid, parallel online monitoring of up to 63 shake flasks.
- For microbial cultivation and cell culture.
- Pre-calibrated cultivation vessels are ready-to-use.
- Compatible with standard shakers.
- Glass & plastic flasks in different sizes available.
- Non-invasive measurement.
- Used in seed train & bioprocess development.
Applications
- Oxygen and pH monitoring in shake flasks (Erlenmeyer flask), cultivation tubes, and T-flasks.
- Media evaluation and optimization.
- Wireless data transfer.
- Compatible with standard shakers.
- For microbial cultivations & cell culture.
The Smart Measurement Method
The sensor spots are fixed at the bottom of the shake flasks, cultivation tubes, or T-flasks. The luminescence of the dyes embedded in these sensor spots is excited by the Shake Flask Reader. The luminescence lifetime is detected non-invasively through the transparent flask. The luminescence lifetime of the sensor dye depends on the oxygen partial pressure and the pH of the sample, respectively. It is converted to oxygen and pH values by the software using the provided calibration data.
The SFR Software
SFRS is the integrated control center for the SFR Shake Flask Reader. In addition, it offers an integrated database to show and evaluate measurements. The connection between PC and SFR is wireless. Oxygen and pH are visualized in real-time during the entire cultivation. Results are displayed in a variety of graphical representations. The advanced graphic data handling supports Design of Experiment. All measured data can be exported to Excel® or as .csv for further evaluation. Furthermore, the current measurement can be compared to stored cultivations online.
Benefits
- Enables process monitoring in shake flasks
- Online Oxygen Uptake Rate monitoring
- Systematic optimization of cultivation parameters
- Compliant with FDA 21 CRF part 11
- Supports finding feeding points
- Facilitates optimization of harvesting strategies
